E2F-4 rabbit pAb

SKU: orb766927

Description

Rabbit polyclonal antibody to E2F-4

Images & Validation

• Tested Applications: ELISA, IHC-P, WB
• Dilution range: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.
• Reactivity: Human, Mouse, Rat

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human E2F4 around the non-acetylation site of Lys96. AA range:61-110
• Molecular Weight: 43
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Anti-E2F4 antibody, anti-Transcription factor E2F4 antibody, anti-E2F-4 antibody

Immunohistochemical analysis of paraffin-embedded rat-kidney, antibody was diluted at 1:100.

Immunohistochemical analysis of paraffin-embedded mouse-kidney, antibody was diluted at 1:100.

Western Blot analysis of Jurkat cells using E2F-4 Polyclonal Antibody. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit.

Immunohistochemical analysis of paraffin-embedded rat-kidney, antibody was diluted at 1:100.