Recombinant TOP2A Antibody / Topoisomerase II alpha

SKU: orb2644374

Description

The topoisomerase II is the predominant mechanism of resistance to several chemotherapeutic agents.

Images & Validation

• Tested Applications: IHC-P
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
• Reactivity: Human
• Application Notes:
  Optimal dilution of the recombinant TOP2A antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: Rabbit IgG
• Clone No.: TOP2A/4397R
• Immunogen: A portion of amino acids 1431-1531 from the human protein was used as the immunogen for the recombinant TOP2A antibody.
• Purification: Protein A affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

IHC staining of FFPE human tonsil with recombinant TOP2A antibody. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human lymph node with recombinant TOP2A antibody. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Quick Database Links

UniProt

P11388