Topoisomerase II alpha Antibody / TOP2A

• Catalog Number: orb1151540
• Category: Antibodies
• Description: Mouse monoclonal antibody to Topoisomerase II alpha
• Species/Host: Mouse
• Clonality: Recombinant
• Clone Number: rTOP2A/6569
• Tested applications: IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: Mouse IgG2a, kappa
• Immunogen: A portion of amino acids 1352-1493 was used as the immunogen for the recombinant TOP2A antibody.
• Antibody Type: Primary Antibody
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml
• Purity: Protein A/G affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This recombinant TOP2A antibody is available for research use only.
• UniProt ID: P11388
• Storage: Aliquot the recombinant TOP2A antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the recombinant TOP2A antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

SDS-PAGE analysis of purified, BSA-free recombinant TTOP2A antibody (clone rTOP2A/6569) as confirmation of integrity and purity.

IHC staining of FFPE human tonsil tissue with recombinant TOP2A antibody (clone rTOP2A/6569). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Negative control: IHC staining of FFPE human brain tissue with recombinant TOP2A antibody (clone rTOP2A/6569) at 2 ug/ml in PBS for 30 min RT. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Western blot testing of human 1) HeLa and 2) Jurkat cell lysate using recombinant TOP2A antibody (clone rTOP2A/6569). Predicted molecular weight ~174 kDa.

IHC staining of FFPE human lymph node with recombinant TOP2A antibody (clone rTOP2A/6569). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.