Topoisomerase II alpha Antibody / TOP2A

• Catalog Number: orb1151540
• Category: Antibodies
• Description: Mouse monoclonal antibody to Topoisomerase II alpha
• Clonality: Recombinant
• Species/Host: Mouse
• Isotype: Mouse IgG2a, kappa
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Purity: Protein A/G affinity
• Immunogen: A portion of amino acids 1352-1493 was used as the immunogen for the recombinant TOP2A antibody.
• UniProt ID: P11388
• Tested applications: IHC-P, WB
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml
• Application notes: Optimal dilution of the recombinant TOP2A antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: rTOP2A/6569
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Storage: Aliquot the recombinant TOP2A antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Hazard Information: This recombinant TOP2A antibody is available for research use only.
• Note: For research use only

SDS-PAGE analysis of purified, BSA-free recombinant TTOP2A antibody (clone rTOP2A/6569) as confirmation of integrity and purity.

IHC staining of FFPE human tonsil tissue with recombinant TOP2A antibody (clone rTOP2A/6569). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Negative control: IHC staining of FFPE human brain tissue with recombinant TOP2A antibody (clone rTOP2A/6569) at 2 ug/ml in PBS for 30 min RT. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Western blot testing of human 1) HeLa and 2) Jurkat cell lysate using recombinant TOP2A antibody (clone rTOP2A/6569). Predicted molecular weight ~174 kDa.

IHC staining of FFPE human lymph node with recombinant TOP2A antibody (clone rTOP2A/6569). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.