Recombinant TOP2A Antibody / Topoisomerase II alpha

SKU: orb2636710

Description

It recognizes a 170kDa protein, which is identified as topoisomerase II is also implicated in drug resistance of tumor cells and has been shown to be over-expressed in many human cancers. Decreased expression of Topo IIa is the predominant mechanism of resistance to several chemotherapeutic agents.

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml
• Reactivity: Human, Mouse
• Application Notes:
  Optimal dilution of the recombinant TOP2A antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Recombinant
• Isotype: Mouse IgG2a, kappa
• Clone No.: rTOP2A/6569
• Immunogen: A portion of amino acids 1352-1493 was used as the immunogen for the recombinant TOP2A antibody.
• Purification: Protein A/G affinity
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

IHC staining of FFPE human lymph node with recombinant TOP2A antibody (clone rTOP2A/6569). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human tonsil tissue with recombinant TOP2A antibody (clone rTOP2A/6569). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Negative control: IHC staining of FFPE human brain tissue with recombinant TOP2A antibody (clone rTOP2A/6569) at 2 ug/ml in PBS for 30 min RT. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Western blot testing of human 1) HeLa and 2) Jurkat cell lysate using recombinant TOP2A antibody (clone rTOP2A/6569). Predicted molecular weight ~174 kDa.

SDS-PAGE analysis of purified, BSA-free recombinant TTOP2A antibody (clone rTOP2A/6569) as confirmation of integrity and purity.

Quick Database Links

UniProt

P11388