TOP1 Antibody (N-term)

SKU: orb1788423

Description

Purified Mouse Monoclonal Antibody (Mab)

Research Area

Infectious Disease & Virology

Images & Validation

• Tested Applications: FC, WB
• Dilution Range: WB: 1:1000, FC: 1:25
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1,κ
• Immunogen: This TOP1 antibody is generated from a mouse immunized with a recombinant protein from the N-terminal region of human TOP1. Antigen Region: 1-290 aa.
• Target: TOP1
• Molecular Weight: 90726 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DNA topoisomerase 1, DNA topoisomerase I, TOP1

Flow cytometric analysis of Hela cells using TOP1 Antibody (N-term) (green) compared to an isotype control of mouse IgG1 (blue). Diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.

Western blot analysis of lysates from MCF-7, Jurkat, PC-12 cell line (from left to right), using TOP1 Antibody (N-term). Diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.

Quick Database Links

Gene Symbol

TOP1

UniProt

P11387