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Catalog Number | orb1936312 |
---|---|
Category | Antibodies |
Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB34034 |
Tested applications | IF, WB |
Predicted Reactivity | Hamster, Mouse, Rat |
Reactivity | Human |
Isotype | Rabbit IgG |
Antibody Type | Primary Antibody |
Dilution range | IF: 1:10~50, WB: 1:1000 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Conjugation | Unconjugated |
MW | 90726 Da |
Target | This TOP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 2-31 amino acids from the N-terminal region of human TOP1. |
UniProt ID | P11387 |
NCBI | NP_003277.1 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | DNA topoisomerase 1, DNA topoisomerase I, TOP1 Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
TOP1 Antibody (N-term) western blot analysis in NCI-H460 cell line lysates (35 ug/lane). This demonstrates the TOP1 antibody detected the TOP1 protein (arrow).
Confocal immunofluorescent analysis of TOP1 Antibody (N-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).