TOP1 Antibody (N-term)

SKU: orb1927221

Description

TOP1 Antibody (N-term)

Research Area

Cancer Biology, Infectious Disease & Virology

Images & Validation

• Tested Applications: FC, WB
• Dilution Range: FC - 1:25, WB - 1:1000
• Reactivity: Human, Mouse, Rat

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1,κ
• Molecular Weight: 90726 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Flow cytometric analysis of Hela cells using TOP1 Antibody (N-term) (green) compared to an isotype control of mouse IgG1 (blue). Diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.

Western blot analysis of lysates from HUVEC, Jurkat, MCF-7, PC-12 cell line (from left to right), using TOP1 Antibody (N-term). Diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35μg per lane.

Quick Database Links

UniProt

P11387