TNFSF11 Antibody

• Catalog Number: orb1240016
• Category: Antibodies
• Description: TNFSF11 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Anti-sRANK-L antibody (orb1240016) was raised against a peptide corresponding to 14 amino acids near the center of human sRANK-L. The immunogen is located within amino acids 140-190 of sRANK-L.
• Concentration: 1 mg/mL
• Dilution range: WB: 0.25-4 μg/mL; IHC: 5 μg/mL.Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in human and rat samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 36kDObserved: 36 kD
• Target: TNFSF11
• UniProt ID: O14788
• NCBI: NP_003692
• Storage: sRANK Ligand antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: sRANK Ligand Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: sRANK Ligand Antibody: ODF, OPGL, sOdf, CD254, OPT
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot Validation in Human Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: sRANK-L orb1240016 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Mouse Tissues. Loading: 15 µg of lysates per lane. Antibodies: sRANK-L orb1240016 (2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation with Recombinant Protein. Loading: 30 ng of human sRANK-L recombinant protein per lane. Antibodies: sRANK-L orb1240016 (A: 1 µg/mL, B: 2 µg/mL and C: 4 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Observed at around 27kD.

Western Blot Validation in Rat Liver Tissue. Loading: 15 µg of lysates per lane. Antibodies: sRANK-L orb1240016 (A: 0.25 µg/mL and B: 0.5 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunohistochemistry Validation of sRANK-L in Human Liver Tissue. Immunohistochemical analysis of paraffin-embedded human liver tissue using anti sRANK-L antibody (orb1240016) at 5 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunohistochemistry and Induced Expression Validation of sRANK-L in Rat Osteogenic Compartment (Hassan et al., 2017). Protein analysis for sRANK-L by immunohistochemistry with anti-sRANK-L antibodies (orb1240016) in rat osteogenic cells. Strong immunoreactivity is shown for sRANK-L in osteogenic cells at 18hr after upper jaw molar extraction as compared to baseline.