TNFSF11 Antibody

SKU: orb1240016

Description

TNFSF11 Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA, IHC-P, WB
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Anti-sRANK-L antibody (orb1240016) was raised against a peptide corresponding to 14 amino acids near the center of human sRANK-L. The immunogen is located within amino acids 140-190 of sRANK-L.
• Target: TNFSF11
• Molecular Weight: Predicted: 36kDObserved: 36 kD
• Purification: sRANK Ligand Antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: sRANK Ligand Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• Disclaimer: For research use only

Alternative Names

sRANK Ligand Antibody: ODF, OPGL, sOdf, CD254, OPTB2, RANKL, TRANCE, hRANKL2, Tumor necrosis factor ligand superfamily member 11, Osteoclast differentiation factor, ODF

Western Blot Validation in Human Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: sRANK-L orb1240016 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Mouse Tissues. Loading: 15 µg of lysates per lane. Antibodies: sRANK-L orb1240016 (2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation with Recombinant Protein. Loading: 30 ng of human sRANK-L recombinant protein per lane. Antibodies: sRANK-L orb1240016 (A: 1 µg/mL, B: 2 µg/mL and C: 4 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Observed at around 27kD.

Western Blot Validation in Rat Liver Tissue. Loading: 15 µg of lysates per lane. Antibodies: sRANK-L orb1240016 (A: 0.25 µg/mL and B: 0.5 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunohistochemistry Validation of sRANK-L in Human Liver Tissue. Immunohistochemical analysis of paraffin-embedded human liver tissue using anti sRANK-L antibody (orb1240016) at 5 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunohistochemistry and Induced Expression Validation of sRANK-L in Rat Osteogenic Compartment (Hassan et al., 2017). Protein analysis for sRANK-L by immunohistochemistry with anti-sRANK-L antibodies (orb1240016) in rat osteogenic cells. Strong immunoreactivity is shown for sRANK-L in osteogenic cells at 18hr after upper jaw molar extraction as compared to baseline.

Quick Database Links

Gene Symbol

TNFSF11

UniProt

O14788

RefSeq (Protein):NP_003692