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RANKL/CD254 Rabbit Polyclonal Antibody

Catalog Number: orb11190

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb11190
CategoryAntibodies
DescriptionRANKL/CD254 Rabbit Polyclonal Antibody
TargetTNFSF11
ClonalityPolyclonal
Species/HostRabbit
IsotypeIgG
ConjugationUnconjugated
ReactivityHuman, Mouse
Predicted ReactivityCanine, Rat
Form/AppearanceLiquid
Concentration1mg/ml
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
ImmunogenKLH conjugated synthetic peptide derived from human OPGL (210-317/317aa)
UniProt IDO14788
RRIDAB_10751983
MW35 kDa
Tested applicationsFC, IF, IHC-Fr, IHC-P, WB
Dilution rangeWB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test
Antibody TypePrimary Antibody
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesOPGL; CD254; hRANKL2; ODF; OPGL; Osteoclast differ
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NoteFor research use only

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Yuxiang Zhan 1, Siqin Zhang 2, Minjie Yang 3, Yuchen Wang 3, Jiaquan Li 4, Xiaolin Nong Artesunate Promotes Bone Remodeling Through TRAF6-Mediated NF-κB Signaling Under Orthodontic Stress in Diabetic Rats Int Dent J, (2025)

Applications

IHC

Reactivity

Rat
RANKL/CD254 Rabbit Polyclonal Antibody

Blank control: mouse splenocytes (blue), Isotype Control Antibody: Rabbit IgG (orange), Secondary Antibody: Goat anti-rabbit IgG-FITC (white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA, Primary Antibody Dilution: 1 µl in 100 µl 1X PBS containing 0.5% BSA (green).

RANKL/CD254 Rabbit Polyclonal Antibody

Sample: HUVEC-C Cell (Human) Lysate at 40 ug, HepG2 Cell (Human) Lysate at 40 ug, Primary: Anti-RANKL/CD254 (orb11190) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 35kD, Observed band size: 56kD.

RANKL/CD254 Rabbit Polyclonal Antibody

Sample: Lymph node (Mouse) Lysate at 40 ug, Thymus (Mouse) Lysate at 40 ug, Primary: Anti-RANKL'CD254 (orb11190) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 37'50 kD, Observed band size: 50 kD.

RANKL/CD254 Rabbit Polyclonal Antibody

Tissue/Cell: human gastric carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-OPGL/RANKL/ODF Polyclonal Antibody, Unconjugated (orb11190) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

RANKL/CD254 Rabbit Polyclonal Antibody

Tissue/Cell: mouse spleen tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-OPGL/RANKL/ODF Polyclonal Antibody, Unconjugated (orb11190) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

  • RANKL/CD254 Rabbit Polyclonal Antibody [orb526532]

    FC,  WB

    Canine, Equine, Rabbit

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    50 μl, 100 μl, 200 μl
  • RANKL/CD254 Rabbit Polyclonal Antibody [orb500861]

    FC,  WB

    Bovine, Canine, Equine, Porcine, Rabbit, Zebrafish

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    50 μl, 100 μl, 200 μl
  • RANKL/CD254 Rabbit Polyclonal Antibody (APC) [orb1005726]

    FC,  IF

    Canine, Rat

    Human, Mouse

    Rabbit

    Polyclonal

    APC

    100 μl
  • RANKL/CD254 Rabbit Polyclonal Antibody (PE) [orb526351]

    FC

    Canine, Equine, Rabbit

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    PE

    100 μl
  • RANKL/CD254 Rabbit Polyclonal Antibody (FITC) [orb526354]

    FC

    Canine, Equine, Rabbit

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    FITC

    100 μl