RANKL/CD254 Rabbit Polyclonal Antibody

SKU: orb11190

Description

RANKL/CD254 Rabbit Polyclonal Antibody

Research Area

Immunology & Inflammation; Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: FC, IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test
• Reactivity: Human, Mouse
• Predicted Reactivity: Canine, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human OPGL (210-317/317aa)
• Target: TNFSF11
• Molecular Weight: 50 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CD254; ODF; OPGL; OPTB2; RANKL; TNLG6B; TRANCE; hRANKL2; sOdf; Ly109l; TNF11_HUMAN; TNFSF11; Osteoclast differentiation factor (ODF); Osteoprotegerin ligand (OPGL); Receptor activator of nuclear factor kappa-B ligand (RANKL); TNF-related activation-induced cytokine (TRANCE); TNF11_MOUSE; TNF superfamily member 11; tumor necrosis factor (ligand) superfamily, member 11

Quick Database Links

Gene Symbol

TNFSF11

UniProt

O14788

Li, Jingwu et al. Effect of gallium nitrate on the expression of osteoprotegerin and receptor activator of nuclear factor‑κB ligand in osteoblasts in vivo and in vitro Mol Med Rep, 13, 769-777 (2016)

PubMed: 26647856

Applications

IHC, WB

Reactivity

Rat

HH Mustafa, SMA Hassan, SA Muhammad Influence of platelet-rich plasma on RANKL and IL-1 immunohistochemical expression in periodontitis-related bone cell proliferation and differentiation The Saudi Dental Journal, (2024)

PubMed: 40952875

Applications

IHC

Reactivity

Rat

Yuxiang Zhan 1, Siqin Zhang 2, Minjie Yang 3, Yuchen Wang 3, Jiaquan Li 4, Xiaolin Nong Artesunate Promotes Bone Remodeling Through TRAF6-Mediated NF-κB Signaling Under Orthodontic Stress in Diabetic Rats Int Dent J, (2025)

PubMed: 40393315

Applications

IHC

Reactivity

Rat

Jiao, Mengyu et al. Osteoprotegerin/receptor activator of nuclear factor-?B ligand are involved in periodontitis-promoted vascular calcification Exp Ther Med, 24, 512 (2022)

PubMed: 35813311

Applications

IHC, WB

Reactivity

Rat