TNF Antibody

• Catalog Number: orb1261231
• Category: Antibodies
• Description: TNF Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: A synthetic peptide of human TNF
• Concentration: batch dependent
• Dilution range: WB: 1:1000 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 21kDa
• Target: TNF
• UniProt ID: P01375
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: Tumor necrosis factor, Cachectin, TNF-alpha, Tumor
• Note: For research use only
• Application notes: WB: 1:1000 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using TNF-α antibody (orb1261231). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunohistochemistry of paraffin-embedded human stomach using TNF-α antibody (orb1261231) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of RAW264. 7 cells using TNF-α antibody (orb1261231) at dilution of 1:100. Blue: DAPI for nuclear staining.