You have no items in your shopping cart.
Description
Research Area
Immunology & Inflammation
Images & Validation
−Item 1 of 3
| Tested Applications | ELISA, WB |
|---|---|
| Reactivity | Mouse |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mTNF-α (murine TNF-alpha). |
| Target | Tnf |
| Purification | Anti-mTNF-α specific antibody was purified by affinity chromatography and then biotinylated. |
| Conjugation | Biotin |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Concentration | batch dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−DIF, Tnfa, TNF-a, TNFSF2, Tnfsf1a, TNFalpha, TNF-alpha, Tnfsf2, Tumor necrosis factor, Cachectin
Similar Products
−- Item 1 of 4
- Item 1 of 1
Rat TNF Receptor-Associated Factor 2 (TRAF2) ELISA Kit [orb1088219]
Rat
78.13-5000 pg/mL
25.4 pg/mL
96 T, 48 T - Item 1 of 1
Human Soluble Tumor Necrosis Factor Receptor Superfamily, Member 1A (sTNFRSF1A) ELISA Kit [orb1146887]
Human
15.63-1000 pg/mL
6.5 pg/mL
96 T, 48 T - Item 1 of 1
Human Soluble Tumor Necrosis Factor Receptor Superfamily, Member 1B (sTNFRSF1B) ELISA Kit [orb1146888]
Human
0.16-10 ng/mL
0.066 ng/mL
96 T, 48 T - Item 1 of 1
Rat Tumor Necrosis Factor Receptor Superfamily, Member 10A (TNFRSF10A) ELISA Kit [orb1146955]
Rat
0.16-10 ng/mL
0.057 ng/mL
48 T, 96 T
Quality Guarantee
Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].
To detect Murine TNF-alpha by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 – 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Anti-Murine TNF-alpha (orb1272287) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Murine TNF-alpha.
To detect Murine TNF-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Murine TNF-alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect Murine TNF-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Murine TNF-alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Documents Download
Datasheet
Product Information
Request a Document
Protocol Information
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Tnf Antibody (Biotin) (orb1272286)
- 0.0
Based on 0 reviews
Participating in our Biorbyt product reviews program enables you to support fellow scientists by sharing your firsthand experience with our products.
Login to Submit a ReviewAvailable Sizes
Select a size below
Free Secondary Antibody (20 ul)0/0
Please add an antibody product to your cart first.
Usually dispatched within 5-10 working days