CD137L / 4-1BBL / TNFSF9 Antibody

• Catalog Number: orb751086
• Category: Antibodies
• Description: Mouse monoclonal antibody against TNFSF9 conjugated to Biotin
• Target: TNFSF9
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG2a
• Conjugation: Biotin
• Reactivity: Human
• Form/Appearance: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Concentration: Purified Ab conjugated to Biotin
• Buffer/Preservatives: Antibody purified from Bioreactor Concentrate by Protein A/G and conjugated to Biotin. Prepared in 10mM PBS with 0.05% rAlbumin and 0.05% azide.
• Purification: Protein A
• Immunogen: Recombinant full-length human CD137L protein
• UniProt ID: P41273
• MW: 27kDa
• Tested applications: FC, IF, IHC
• Dilution range: Flow Cytometry (2-4ug/million cells in 0.1ml); Immunofluorescence (2-4ug/ml);,Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
• Application notes: Positive Control: Raji, 293T or HEK293 cells. Human renal cell carcinoma or lung carcinoma.
• Clone Number: CD137L/1547
• Expression System: Bioreactor
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-4-1BB ligand antibody, anti-4-1BBL antibody,
• Research Area: Immunology & Inflammation, Signal Transduction
• Note: For research use only
• Entrez: 8744

Flow Cytometric Analysis of PFA-fixed HEK293 cells using CD137L-Monospecific Mouse Monoclonal Antibody (CD137L/1547) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with CD137L-Monospecific Mouse Monoclonal Antibody (CD137L/1547).

SDS-PAGE Analysis Purified CD137L-Monospecific Mouse Monoclonal Antibody (CD137L/1547). Confirmation of Purity and Integrity of Antibody.