CD40 Ligand / CD154 / TRAP1 (Activation Marker of T-Lymphocytes) Antibody

SKU: orb751205

Description

Mouse monoclonal antibody against CD40LG conjugated to Biotin

Images & Validation

• Tested Applications: ELISA, FC, IF, IHC, WB
• Dilution range: ELISA (For coating, order antibody without BSA); Flow Cytometry (2-4ug/million cells); ,Immunofluorescence (2-4ug/ml); Western Blot (2-4ug/ml);,Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
• Reactivity: Human
• Application Notes:
  Positive Control: Jurkat cells. Tonsil, Spleen or Thymus

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone No.: CD40LG/2761
• Immunogen: Recombinant fragment (around aa 108-261) of human CD40LG protein (exact sequence is proprietary)
• Target: CD40LG
• Source/Expression System: Bioreactor
• Molecular Weight: 36kDa
• Purification: Protein A

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Buffer/Preservatives: Antibody purified from Bioreactor Concentrate by Protein A/G and conjugated to Biotin. Prepared in 10mM PBS with 0.05% rAlbumin and 0.05% azide.
• Concentration: Purified Ab conjugated to Biotin
• Disclaimer: For research use only

Alternative Names

Anti-CD154 antibody, anti-gp39 antibody, anti-hCD40L antibody, anti-HIGM1 antibody, anti-IGM antibody, anti-IMD3 antibody, anti-T B cell activating molecule antibody, anti-T BAM antibody, anti-T-cell antigen Gp39 antibody, anti-TNF-related activation protein antibody, anti-TrAP antibody, anti-Tumor necrosis factor ligand superfamily member 5 (TNFSF5) antibody

Analysis of Protein Array containing more than 19000 full-length human proteins using CD40-Ligand Mouse Monoclonal Antibody (CD40LG/2761) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SDÂ’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDÂ’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Flow Cytometric Analysis of Jurkat cells using CD40L-Monospecific Mouse Monoclonal Antibody (CD40LG/2761) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Immunofluorescent staining of paraformaldehyde-fixed Jurkat cells using CD40L Mouse Monoclonal Antibody (CD40LG/2761) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Reddot.

SDS-PAGE Analysis Purified CD40L Mouse Monoclonal Antibody (CD40LG/2761). Confirmation of Purity and Integrity of Antibody.

Quick Database Links

Gene Symbol

CD40LG

UniProt

P29965

NCBI Gene ID

959