CD40 Ligand / CD154 / TRAP1 (Activation Marker of T-Lymphocytes) Antibody

• Catalog Number: orb751205
• Category: Antibodies
• Description: Mouse monoclonal antibody against CD40LG conjugated to Biotin
• Target: CD40LG
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG2b
• Conjugation: Biotin
• Reactivity: Human
• Form/Appearance: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Concentration: Purified Ab conjugated to Biotin
• Buffer/Preservatives: Antibody purified from Bioreactor Concentrate by Protein A/G and conjugated to Biotin. Prepared in 10mM PBS with 0.05% rAlbumin and 0.05% azide.
• Purification: Protein A
• Immunogen: Recombinant fragment (around aa 108-261) of human CD40LG protein (exact sequence is proprietary)
• UniProt ID: P29965
• MW: 36kDa
• Tested applications: ELISA, FC, IF, IHC, WB
• Dilution range: ELISA (For coating, order antibody without BSA); Flow Cytometry (2-4ug/million cells); ,Immunofluorescence (2-4ug/ml); Western Blot (2-4ug/ml);,Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
• Application notes: Positive Control: Jurkat cells. Tonsil, Spleen or Thymus
• Clone Number: CD40LG/2761
• Expression System: Bioreactor
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-CD154 antibody, anti-gp39 antibody, anti-hCD4
• Research Area: Cardiovascular Research, Cell Biology, Immunology
• Note: For research use only
• Entrez: 959
• Expiration Date: 12 months from date of receipt.

Analysis of Protein Array containing more than 19000 full-length human proteins using CD40-Ligand Mouse Monoclonal Antibody (CD40LG/2761) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SDÂ’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDÂ’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Flow Cytometric Analysis of Jurkat cells using CD40L-Monospecific Mouse Monoclonal Antibody (CD40LG/2761) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Immunofluorescent staining of paraformaldehyde-fixed Jurkat cells using CD40L Mouse Monoclonal Antibody (CD40LG/2761) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Reddot.

SDS-PAGE Analysis Purified CD40L Mouse Monoclonal Antibody (CD40LG/2761). Confirmation of Purity and Integrity of Antibody.