CD40 Ligand / CD154 / TRAP1 (Activation Marker of T-Lymphocytes) Antibody

• Catalog Number: orb751205
• Category: Antibodies
• Description: Mouse monoclonal antibody against CD40LG conjugated to Biotin
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: CD40LG/2761
• Tested applications: ELISA, FC, IF, IHC, WB
• Reactivity: Human
• Isotype: IgG2b
• Immunogen: Recombinant fragment (around aa 108-261) of human CD40LG protein (exact sequence is proprietary)
• Concentration: Purified Ab conjugated to Biotin
• Dilution range: ELISA (For coating, order antibody without BSA); Flow Cytometry (2-4ug/million cells); ,Immunofluorescence (2-4ug/ml); Western Blot (2-4ug/ml);,Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
• Form/Appearance: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Conjugation: Unconjugated
• MW: 36kDa
• Target: CD40LG
• Entrez: 959
• UniProt ID: P29965
• Expression System: Bioreactor
• Storage: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
• Buffer/Preservatives: Antibody purified from Bioreactor Concentrate by Protein A/G and conjugated to Biotin. Prepared in 10mM PBS with 0.05% rAlbumin and 0.05% azide.
• Alternative names: Anti-CD154 antibody, anti-gp39 antibody, anti-hCD4
• Note: For research use only
• Application notes: Positive Control: Jurkat cells. Tonsil, Spleen or Thymus
• Expiration Date: 12 months from date of receipt.