TLR4 antibody

• Catalog Number: orb535107
• Category: Antibodies
• Description: TLR4 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the n-terminus of human TLR4 protein.
• Concentration: 1.0 mg/ml
• Dilution range: ELISA: 5 µg/ml, IHC: 1:100 - 1:200, WB: 1:1000
• Form/Appearance: Liquid (sterile filtered)
• Purity: This affinity purified antibody is directed against human TLR4 protein. The product was purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest reactivity with TLR4 protein from human based on 100% homology with the immunizing sequence. The analysis also shows 100% cross-reactivity with gorilla and bonobo, and canine species.
• Conjugation: Unconjugated
• UniProt ID: O00206
• NCBI: 19924149
• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: Rabbit Anti-TLR4 Antibody, ARMD10 antibody, CD284
• Note: For research use only
• Application notes: Anti-TLR4 affinity purified antibody has been tested for use in ELISA, Immunohistochemistry, and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 96 kDa in size corresponding to TLR4 protein in overexpressed lysate by western blotting in the appropriate cell lysate or extract.
• Expiration Date: 12 months from date of receipt.