TLR4 Antibody

SKU: orb535107

Description

TLR4 antibody

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 5 ug/ml, IHC: 1:100 - 1:200, WB: 1:1000
• Reactivity: Human
• Application Notes:
  Anti-TLR4 affinity purified antibody has been tested for use in ELISA, Immunohistochemistry, and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 96 kDa in size corresponding to TLR4 protein in overexpressed lysate by western blotting in the appropriate cell lysate or extract.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the n-terminus of human TLR4 protein.
• Target: TLR4
• Purity: This affinity purified antibody is directed against human TLR4 protein. The product was purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest reactivity with TLR4 protein from human based on 100% homology with the immunizing sequence. The analysis also shows 100% cross-reactivity with gorilla and bonobo, and canine species.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/ml
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

Rabbit Anti-TLR4 Antibody, ARMD10 antibody, CD284 antibody, Toll (Drosophila) homolog antibody, HToll, Homolog of Drosophila Toll, TLR-4 Antibody, TOLL antibody, Toll Endotoxin Hyporesponsiveness antibody, Toll like receptor 4 antibody, Toll like receptor 4 precursor antibody

Immunohistochemistry of Rabbit Anti-TLR4 Antibody. Tissue: Human Tonsil. Antigen retrieval: HIER using Citrate Buffer for 20 min. Primary Antibody: Anti-TLR4 at 1:100 at RT for 30 mins. Secondary Antibody: Ready-to-Use Anti-Rabbit Poly-HRP-IgG at RT for 8mins. Stain: DAB. Counterstain: Hematoxylin. Light cytoplasmic staining in random cells near blood vessels is consistent with what is expected of TLR staining in the tonsil.

Western Blot of Rabbit Anti-TLR4 Antibody. Lane 1: Opal Pre-Stained Molecular Weight Ladder. Lane 2: Recombinant TLR4 (Myc/DDK tag). Blocking: BlockOut buffer (p/n orb348644). Primary Antibody: Anti-TLR4 at 1:1000 overnight at 4°C. Secondary Antibody: Goat Anti-Rabbit IgG HRP conjugated (p/n orb347654) at 1:70000 for 30 min RT. Expect: ~50kDa endogenous, ~93kDa overexpressed. Notes: Highly glycosylated protein.

Quick Database Links

Gene Symbol

TLR4

UniProt

O00206

RefSeq:19924149