TLR4 Rabbit Polyclonal Antibody

• Catalog Number: orb11489
• Category: Antibodies
• Description: TLR4 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Sheep
• Reactivity: Human, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from rat TLR4 (751-835/835aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100, IF=1:100-500, Flow-Cyt=2ug/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 110 kDa
• Target: TLR4
• UniProt ID: Q9QX05
• RRID: AB_10748590
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: TLR-4; TLR 4; ARMD10; CD 284; CD284; CD284 antigen
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Colak, Ozlem et al. Evaluation of Safe Systemic Immunosuppression Created with Dexamethasone in Prevention of Capsular Contracture: A Glance to Distinct Perspectives with Toll-Like Receptors Aesthetic Plast Surg, 42, 1133-1143 (2018)

PubMed: 29564486

Applications

IHC

Reactivity

Rat

Leopoldo Cosme-Silva a 1 , Renan Dal-Fabbro b 1 , Fernanda de Lima Pontes a , Leticia Cabrera Capalbo c , Edilson Ervolino d , Luciano Tavares Angelo Cintra d , Juan José Segura-Egea e , João Eduardo Gomes Filho Multi-Strain Probiotic Formula Modulates Expression of β-Defensin-2, β-Defensin-3, and TLR-4 in Male Rats with Apical Periodontitis Archives of Oral Biology, (2024)

PubMed:

Applications

IHC

Reactivity

Rat

David Zapico 1 2, José Espinosa 1 2, Miguel Criado 1 2, Daniel Gutiérrez 1 2, María Del Carmen Ferreras 1 2, Julio Benavides 2, Valentín Pérez 1 2, Miguel Fernández Immunohistochemical expression of TLR1, TLR2, TLR4, and TLR9 in the different types of lesions associated with bovine paratuberculosis Vet Pathol ., (2024)

PubMed: 39720873

Effects of Bdellovibrio bacteriovorus HD100 on experimental periodontitis in rats Mol Oral Microbiol, 38, 158-170 (2023)

PubMed: 36495122

Applications

IHC

Reactivity

Rat

Ostadhadi, Sattar et al. Mesenteric artery responsiveness to acetylcholine and phenylephrine in cirrhotic rats challenged with endotoxin: the role of TLR4 Can J Physiol Pharmacol, 93, 475-483 (2015)

PubMed: 25978623

Applications

IHC

Reactivity

Rat

Blank control: K562. Primary Antibody (green line): Rabbit Anti-TLR4 antibody (orb11489), Dilution: 0.5 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488, Dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Blank control: Raw264.7. Primary Antibody (green line): Rabbit Anti-TLR4 antibody (orb11489), Dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF488, Dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

HL60 and MCF-7 cells were stained with rabbit polyclonal antibody against TLR4 with two dilutions (1:100 and 1:250). 2nd antibody without primary antibody was used as control included here.

Paraformaldehyde-fixed, paraffin embedded (Human esophageal cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (TLR4) Polyclonal Antibody, Unconjugated (orb11489) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Human MDA-MB-231 cell lysates, Lane 2: Human HUVEC cell lysates, Lane 3: Human HL-60 cell lysates, Primary: Anti-TLR4 (orb11489) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 90 kDa, Observed band size: 120 kDa.