TLR4 Antibody

SKU: orb67563

Description

Rabbit Anti-Human TLR4 Polyclonal

Research Area

Cardiovascular Research, Immunology & Inflammation

Images & Validation

• Tested Applications: FACS, FC, ICC, IF, IHC, WB
• Dilution range: WB (1:500), IHC-P (1:50)
• Reactivity: Human, Mouse
• Application Notes:
  2 µg/ml of SPC-200 was sufficient for detection of TLR4 in 100 ng of partial recombinant mouse TLR4 protein by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Developed against a synthetic peptide corresponding to amino acids 420-435 of human TLR4
• Target: TLR4
• Molecular Weight: 75-80kDa
• Purification: Protein A Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

ARMD10, CD284, TLR 4, TOLL, Toll like receptor 4

Immunohistochemistry analysis using Rabbit Anti-TLR4 Polyclonal Antibody. Tissue: colon colitis. Species: Mouse. Fixation: Formalin. Primary Antibody: Rabbit Anti-TLR4 Polyclonal Antibody at 1:100000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Rabbit at 1:2000 for 1 hour at RT. Counterstain: Methyl Green at 200uL for 2 min at RT. Localization: Inflammatory cells.

Western blot analysis of Mouse brain showing detection of ~75-80kDa TLR4 protein using Rabbit Anti-TLR4 Polyclonal Antibody. Lane 1: Molecular Weight Ladder. Lane 2: Mouse Brain. Load: 15 μg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-TLR4 Polyclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:4000 for 1 hour at RT. Predicted/Observed Size: ~75-80kDa. Other Band (s): ~130kDa (TLR4+MD-2).

Flow Cytometry analysis using Rabbit Anti-TLR4 Polyclonal Antibody. Tissue: Monocytic Leukemia cells (THP-1). Species: Human. Fixation: 2% Formaldehyde for 10 min at RT. Primary Antibody: Rabbit Anti-TLR4 Polyclonal Antibody at 2 μg/106 cells for 60 min at 37°C. Secondary Antibody: Goat Anti-Rabbit Dylight 488 at 1:200 for 40 min at 37°C.

Immunohistochemistry analysis using Rabbit Anti-TLR4 Polyclonal Antibody. Tissue: spleen tissue. Species: Mouse. Primary Antibody: Rabbit Anti-TLR4 Polyclonal Antibody at 1:100.

Western blot analysis of Human HeLa showing detection of ~75-80kDa TLR4 protein using Rabbit Anti-TLR4 Polyclonal Antibody. Lane 1: Molecular Weight Ladder. Lane 2: Human HeLa. Load: 10 μg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-TLR4 Polyclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:4000 for 1 hour at RT. Predicted/Observed Size: ~75-80kDa. Other Band (s): ~60kDa (observed degradation bands (PMID: 16885150).

Immunohistochemistry analysis using Rabbit Anti-TLR4 Polyclonal Antibody. Tissue: Spleen tissue. Species: Mouse. Primary Antibody: Rabbit Anti-TLR4 Polyclonal Antibody at 1:100.

Quick Database Links

Gene Symbol

TLR4

UniProt

O00206

NCBI Gene ID

7099

RefSeq (Protein):NP_612564.1