TLR4 Antibody

SKU: orb535106

Description

TLR4 antibody

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 5 ug/ml, IHC: 1:100 - 1:200, WB: 1:1000
• Reactivity: Human
• Application Notes:
  Anti-TLR4 affinity purified antibody has been tested for use in ELISA, Immunohistochemistry, and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 96 kDa in size corresponding to TLR4 protein in overexpressed lysate by western blotting in the appropriate cell lysate or extract.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the n-terminus of human TLR4 protein.
• Target: TLR4
• Purity: This affinity purified antibody is directed against human TLR4 protein. The product was purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest reactivity with TLR4 protein from human based on 100% homology with the immunizing sequence. The analysis also shows 100% cross-reactivity with gorilla and bonobo, and canine species.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/ml
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

Rabbit Anti-TLR4 Antibody, ARMD10 antibody, CD284 antibody, Toll (Drosophila) homolog antibody, HToll, Homolog of Drosophila Toll, TLR-4 Antibody, TOLL antibody, Toll Endotoxin Hyporesponsiveness antibody, Toll like receptor 4 antibody, Toll like receptor 4 precursor antibody

Immunohistochemistry of Rabbit Anti-TLR4 Antibody. Tissue: Human Tonsil. Antigen retrieval: HIER using Citrate Buffer for 20 min. Primary Antibody: Anti-TLR4 at 1:100 at RT for 30 mins. Secondary Antibody: Ready-to-Use Anti-Rabbit Poly-HRP-IgG at RT for 8mins. Stain: DAB. Counterstain: Hematoxylin. Light cytoplasmic staining in random cells near blood vessels is consistent with what is expected of TLR staining in the tonsil.

Western Blot of Rabbit Anti-TLR4 Antibody. Lane 1: Opal Pre-Stained Molecular Weight Ladder. Lane 2: Recombinant TLR4 (Myc/DDK tag). Blocking: BlockOut buffer (p/n orb348644). Primary Antibody: Anti-TLR4 at 1:1000 overnight at 4°C. Secondary Antibody: Goat Anti-Rabbit IgG HRP conjugated (p/n orb347654) at 1:70000 for 30 min RT. Expect: ~50kDa endogenous, ~93kDa overexpressed. Notes: Highly glycosylated protein.

Quick Database Links

Gene Symbol

TLR4

UniProt

O00206

RefSeq:19924149