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Catalog Number | orb632832 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to ZO-1 |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, FC, IF, IHC, IP, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | tight junction protein 1(zona occludens 1) |
Antibody Type | Primary Antibody |
Dilution range | WB: 1:200-1:2000; IP: 1:200-1:2000; IHC: 1:50-1:200; IF: 1:20-1:200 |
Form/Appearance | liquid |
Purity | ≥95% as determined by SDS-PAGE |
Conjugation | Unconjugated |
MW | 195 kDa |
Target | TJP1 |
UniProt ID | Q07157 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | Tight junction protein 1, Tight junction protein Z Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
HepG2 cells were subjected to SDS PAGE followed by western blot with orb632832 (ZO1 antibody) at dilution of 1:500.
Immunofluorescent analysis of MCF7 cells using orb632832 (ZO-1 Antibody) at dilution of 1:100.
IP result of anti-ZO-1 (IP: orb632832, 4 ug; Detection: orb632832 1:500) with HEK-293 cells lysate 2320 ug.
Immunohistochemistry of paraffin-embedded human kidney using orb632832 (ZO1 antibody) at dilution of 1:100.
IF, IHC-Fr, IHC-P, WB | |
Bovine, Canine, Donkey, Equine, Feline, Gallus, Goat, Guinea pig, Hamster, Human, Mouse, Other, Porcine, Primate, Rabbit, Rat, Sheep | |
Goat | |
Polyclonal | |
Unconjugated |
WB | |
Bovine, Canine, Gallus, Goat, Guinea pig, Mouse, Porcine, Rat, Sheep | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |