Tissue Factor / CD142 extracellular

• Catalog Number: orb1294263
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Tissue Factor
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human
• Isotype: Rabbit-IgG
• Immunogen: Synthetic peptide / Extracellular domain corresponding to Human Tissue Factor / CD142
• Dilution range: Western Blot 1:1000-1:2000 Immunofluorescence 1:300-1:600 Immunohistochemistry (Paraffin) 1:100–1:300
• Conjugation: Unconjugated
• Target: Tissue Factor / CD142 extracellular
• Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Anti-Tissue Factor/CD142 antibody - at 1/1000 dilution. Lysates at 30 μg per lane. This blot was produced using a 10% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294263 overnight at 4°C.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Tissue Factor/CD142 with orb1294263 at 1/100.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Tissue Factor/CD142 with orb1294263 at 1/100.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Tissue Factor/CD142 with orb1294263 at 1/100.

Immunofluorescence: cells were fixed with 4% PFA for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at RT. Cells were stained with orb1294263 anti-CD142 antibody (red) at 1:400 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Product: Tissue Factor/CD142 antibody; Cell: A431; Tissue Factor/CD142 antibody: 1-100.