Tissue Factor Rabbit Polyclonal Antibody

SKU: orb1294263

Description

Rabbit polyclonal antibody to Tissue Factor

Research Area

Cardiovascular Research

Images & Validation

• Tested Applications: IF, IHC, WB
• Dilution Range: Western Blot 1:1000-1:2000, Immunofluorescence 1:300-1:600, Immunohistochemistry (Paraffin) 1:100–1:300
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit-IgG
• Immunogen: Synthetic peptide / Extracellular domain corresponding to Human Tissue Factor / CD142
• Target: Tissue Factor / CD142 extracellular
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300
• Disclaimer: For research use only

anti-Tissue Factor / CD142 antibody - at 1/1000 dilution. Lysates at 30 µg per lane. This blot was produced using a 10% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294263 overnight at 4°C.

Application: Immunocytochemistry (ICC), Sample: Mixture cell, Antibody: Tissue Factor / CD142 extracellular antibody (orb1294263).

Immunofluorescence: cells were fixed with 4% PFA for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at RT. Cells were stained with orb1294263 anti-CD142 antibody (red) at 1:400 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Tissue Factor / CD142 with orb1294263 at 1/100.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Tissue Factor / CD142 with orb1294263 at 1/100.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Tissue Factor / CD142 with orb1294263 at 1/100.

Tissue Factor / CD142 antibody, Cell: A431, Tissue Factor / CD142 antibody: 1-100.

Quick Database Links

Gene Symbol

Tissue Factor / CD142 extracellular