Fibronectin/FN1 Rabbit Polyclonal Antibody

SKU: orb10665

Description

Fibronectin/FN1 Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:200-800
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Fibronectin/Ugl-Y3 (1201-1300/2386aa)
• Target: FN1
• Molecular Weight: 259 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

CIG; Cold insoluble globulin; DKFZp686F10164; DKFZp686H0342; DKFZp686I1370; DKFZp686O13149; ED B; ED-B; fibronectin 1; FINC; FN 1; FN; FN1; FNZ; GFND; GFND2; LETS; Migration stimulating factor; MSF; Transformation sensitive protein; FINC_HUMAN; Fibronectin; Cold-insoluble globulin; Ugl-Y3.

Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Fibronectin) Polyclonal Antibody, Unconjugated (orb10665) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation: Anti-Fibronectin Polyclonal Antibody, conjugated 1:400, 1.5 hours at 37°C, DAPI (5 ug/ml, blue) was used to stain the cell nuclei.

Tissue/Cell: human kidney tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Fibronectin/FN1 Polyclonal Antibody, Unconjugated (orb10665) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/Cell: rat intestine tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Fibronectin/FN1 Polyclonal Antibody, Unconjugated (orb10665) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

FN1

UniProt

P02751

References

Yu-Chi Pan, Sheng-Hua Wu, Hung-Pei Tsai, Ming-Hong Tai, Chien-Ju Lin, Hung-Wei Yang, Shu-Hung Huang Irisin-Loaded Dissolving Microneedles Promote Rapid Wound Healing Advanced NanoBiomed Research, (2025)

PubMed:

Applications

IHC

Reactivity

Rat

Rana Mohammad Taghi Kashi 1, Azadeh Hekmat 1, Saeed Hesami Tackallou 2, Hakimeh Zali 3 Fabrication of a fibrillar β-lactoglobulin-Mumijo-nanohydroxyapatite complex for antibacterial and wound healing applications J Biomater Sci Polym Ed, (2025)

PubMed: 40588417