TIGIT Antibody

• Catalog Number: orb1537007
• Category: Antibodies
• Description: TIGIT Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 3B5
• Tested applications: ELISA, FC, ICC, IF, IHC-P
• Reactivity: Human
• Isotype: IgG2a
• Immunogen: The extracellular domain of human TIGIT
• Concentration: 1 mg/ml
• Dilution range: ELISA, Flo, ICC, IF, IHC-P (10 µg/ml)
• Conjugation: Unconjugated
• Target: TIGIT
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS, 0.02% Sodium Azide, 50% Glycerol. PBS, 0.02% Sodium Azide, 50% Glycerol
• Alternative names: TIGIT, VSTM3, WUCAM, VSIG9
• Note: For research use only
• Application notes: Further information: TIGIT antibody can be used for immunohistochemistry starting at 2 ug/mL. For immunofluorescence start at 1 ug/mL. For flow cytometry at 1 ug/ml. For immunocytochemistry at 1 ug/mL. Antibody validated: Immunohistochemistry in human samples; Immunocytochemistry in human samples; Immunofluorescence in human samples and Flow Cytometry in human samples. All other applications and species not yet tested. Western Blot: Predicted: 26 kDa Observed: 47 kDa
• Expiration Date: 12 months from date of receipt.

Immunohistochemistry of TIGIT in human stomach carcinoma tissue using TIGIT Antibody and control mouse IgG (corner box) at 2 ug/ml.

Immunocytochemistry of TIGIT in over expressing HEK293 cells using TIGIT antibody and control mouse IgG antibody (left corner box) at 1 ug/ml.

Immunofluorescence of TIGIT in human stomach carcinoma tissue using TIGIT Antibody at 5 ug/ml. Green: TIGIT Antibody [3B5] Blue: DAPI staining

Flow cytometry analysis of TIGIT over expressing HEK293 cells using TIGIT antibody at 1 ug/ml. Blue: untransfected HEK293 cells. Yellow: TIGIT over expressing HEK293 cells.