TIGIT Antibody [4A10]

SKU: orb1240137

Description

TIGIT Antibody [4A10]

Images & Validation

• Tested Applications: ELISA, FC, ICC, IF, IHC-P, WB
• Reactivity: Human
• Application Notes:
  TIGIT antibody can be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 1 μg/mL. For flow cytometry at 1 μg/ml. For immunocytochemistry at 1 μg/mL. For Western blot at 1 μg/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples; Immunofluorescence in human samples and Flow Cytometry in human samples. All other applications and species not yet tested.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: 4A10
• Immunogen: TIGIT antibody was raised against the extracellular domain of human TIGIT
• Target: TIGIT
• Molecular Weight: Predicted: 26 kDa Observed: 47 kDa
• Purification: TIGIT Antibody is supplied as protein A purified IgG1.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: TIGIT Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.
• Concentration: 1 mg/mL
• Disclaimer: For research use only

Alternative Names

TIGIT Antibody: T-cell immunoreceptor with Ig and ITIM domains, VSIG9, VSTM3, WUCAM

Western blot analysis of TIGIT in over expressing HEK293 cells using orb1240137 antibody at (A) 0.25 μg/ml, (B) 0.5 μg/ml, and (C) 1 μg/ml.

Immunocytochemistry of TIGIT in over expressing HEK293 cells using TIGIT antibody and control mouse IgG antibody (left corner box) at 1 μg/ml.

Immunofluorescence of TIGIT in over expressing HEK293 cells using TIGIT Antibody at 1 μg/ml. Green: TIGIT Antibody [4A10] (orb1240137) Blue: DAPI staining

Immunofluorescence of TIGIT in human stomach carcinoma tissue using TIGIT Antibody at 5 μg/ml. Green: TIGIT Antibody [4A10] (orb1240137) Blue: DAPI staining

Immunohistochemistry of TIGIT in human stomach carcinoma tissue using TIGIT Antibody and control mouse IgG (corner box) at 2 μg/ml.

Flow cytometry analysis of TIGIT over expressing HEK293 cells using TIGIT antibody at 1 μg/ml. Blue: untransfected HEK293 cells. Yellow: TIGIT over expressing HEK293 cells.

Titration curve analysis of TIGIT mAbs to detect recombinant TIGIT in ELISA with orb1240147, orb1240136, orb1240137, orb1240140, orb1240146 and orb1240115 antibodies at decreasing concentrations.

Quick Database Links

Gene Symbol

TIGIT

UniProt

Q495A1

RefSeq (Protein):NP_776160