TIGIT Antibody [4A11]

• Catalog Number: orb1240140
• Category: Antibodies
• Description: TIGIT Antibody [4A11]
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 4A11
• Tested applications: ELISA, FC, ICC, IF, IHC-P, WB
• Reactivity: Human
• Isotype: IgG1
• Immunogen: TIGIT antibody was raised against the extracellular domain of human TIGIT
• Antibody Type: Primary Antibody
• Concentration: 1 mg/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 26 kDa Observed: 47 kDa
• Target: TIGIT
• UniProt ID: Q495A1
• NCBI: NP_776160
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: TIGIT Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.
• Alternative names: TIGIT Antibody: T-cell immunoreceptor with Ig and
• Note: For research use only
• Application notes: TIGIT antibody can be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 1 μg/mL. For flow cytometry at 1 μg/ml. For immunocytochemistry at 1 μg/mL. For Western blot at 1 μg/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples; Immunofluorescence in human samples and Flow Cytometry in human samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of TIGIT in over expressing HEK293 cells using orb1240140 antibody at (A) 0.25 μg/ml, (B) 0.5 μg/ml, and (C) 1 μg/ml.

Immunocytochemistry of TIGIT in over expressing HEK293 cells using TIGIT antibody and control mouse IgG antibody (left corner box) at 1 μg/ml.

Immunofluorescence of TIGIT in over expressing HEK293 cells using TIGIT Antibody at 1 μg/ml. Green: TIGIT Antibody [4A11] (orb1240140) Blue: DAPI staining

Immunofluorescence of TIGIT in human stomach carcinoma tissue using TIGIT Antibody at 5 μg/ml. Green: TIGIT Antibody [4A11] (orb1240140) Blue: DAPI staining

Immunohistochemistry of TIGIT in human stomach carcinoma tissue using TIGIT Antibody and control mouse IgG (corner box) at 2 μg/ml.

Flow cytometry analysis of TIGIT over expressing HEK293 cells using TIGIT antibody at 1 μg/ml. Blue: untransfected HEK293 cells. Yellow: TIGIT over expressing HEK293 cells.

Titration curve analysis of TIGIT mAbs to detect recombinant TIGIT in ELISA with orb1240147, orb1240136, orb1240137, orb1240140, orb1240146 and orb1240115 antibodies at decreasing concentrations.

A sandwich ELISA was performed using the anti-TIGIT mAb orb1240140 (5 μg/ml) as the capture antibody. Biotin-labeled anti-TIGIT mAb orb1238175 (1 μg/ml) and streptavidin-HRP (0.1 μg/ml) were used for detection. Detection range is from 10 ng to 40 pg.