TIA1 Antibody

• Catalog Number: orb1260872
• Category: Antibodies
• Description: TIA1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 300 to the C-terminus of human TIA1 (NP_071505.2).
• Antibody Type: Primary Antibody
• Concentration: batch dependent
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 43-50kDa
• Target: TIA1
• UniProt ID: P31483
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Alternative names: TIA1, WDM, TIA-1
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using TIA1 antibody (orb1260872) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Immunohistochemistry of paraffin-embedded human colon carcinoma using TIA1 antibody (orb1260872) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human stomach using TIA1 antibody (orb1260872) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of U2OS cells using TIA1 antibody (orb1260872) at dilution of 1:100. Blue: DAPI for nuclear staining.