SYT1 Antibody

• Catalog Number: orb1248965
• Category: Antibodies
• Description: SYT1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Bovine, Canine, Mouse, Rat
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-RDLQSAEKEEQEK
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:2000.Western Blot:Approx. 65kDa band observed in Human Brain (hippocampus) and Mouse and Rat Brain lysates (calculated MW of 47.6kDa according to NP_005630.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Matthew et al, J Cell Biol. 1981 Oct;91(1):257-69.; PMID: 7298720). Recommended concentration: 0.3-1ug/ml.Immunohistochemistry:In paraffin embedded Human Brain shows vesicular staining in neuronal cell and axon. Recommended concentration: 4-6ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: SYT1
• UniProt ID: P21579
• NCBI: NP_005630.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: synaptotagmin I , DKFZp781D2042, SYT1, P65, SVP65,
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:2000.Western Blot:Approx. 65kDa band observed in Human Brain (hippocampus) and Mouse and Rat Brain lysates (calculated MW of 47.6kDa according to NP_005630.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Matthew et al, J Cell Biol. 1981 Oct;91(1):257-69.; PMID: 7298720). Recommended concentration: 0.3-1ug/ml.Immunohistochemistry:In paraffin embedded Human Brain shows vesicular staining in neuronal cell and axon. Recommended concentration: 4-6ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1248965 (1 ug/ml) staining of Human Hippocampus lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1248965 (3.8 ug/ml) staining of paraffin embedded Human Brain. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.