Synaptotagmin 1 Rabbit Polyclonal Antibody

• Catalog Number: orb4362
• Category: Antibodies
• Description: Synaptotagmin 1 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Gallus, Porcine, Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Synaptotagmin 1 (165-260/422aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 48 kDa
• Target: SYT1
• UniProt ID: P21579
• RRID: AB_10931167
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: DKFZp781D2042; P65; SVP65; Synaptotagmin I; SYT1_H
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Sample: Cerebrum (Mouse) Lysate at 40 ug, Cerebellum (Mouse) Lysate at 40 ug, Cerebral cortex (Mouse) Lysate at 40 ug, Cerebrum (Rat) Lysate at 40 ug, Primary: Anti-Synaptotagmin 1 (orb4362) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 48 kD, Observed band size: 53kD.

Sample: Lane 1: Human HepG2 cell lysates, Lane 2: Human A673 cell lysates, Lane 3: Human HeLa cell lysates, Primary: Anti-Synaptotagmin 1 (orb4362) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 48 kDa, Observed band size: 60 kDa.