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Description
Research Area
Cancer Biology, Cardiovascular Research, Cell Biology, Disease Biomarkers, Neuroscience, Signal Transduction
Images & Validation
−Item 1 of 1
| Tested Applications | ICC, IF, IHC, WB |
|---|---|
| Dilution Range | WB (1:1000), IHC-P (1:1000), ICC/IF (1:100) |
| Reactivity | Hamster, Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone No. | N289/16 (Formerly sold as S289-16) |
| Immunogen | Fusion protein amino acids 1548-1582 (cytoplasmic C-terminus) of rat SUR1 |
| Target | SUR1 |
| Molecular Weight | 160kDa |
| Purification | Protein G Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS pH 7.4, 50% glycerol, 0.09% sodium azide. Storage buffer changes when conjugated. |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−AM60008PU-N, ABC36, Abcc8, ATP binding cassette sub family C member 8, HHF1, HRINS, MRP8, PHHI, SUR, SUR1, Sulfonylurea receptor (hyperinsulinemia), ATP binding cassette sub family C (CFTR/MRP) member 8, ATP binding cassette transporter sub family C member 8 (1), ATP-binding cassette sub-family C member 8, HI, SUR1delta2, TNDM2
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Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SUR1 Monoclonal Antibody, Clone N289/16. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-SUR1 Monoclonal Antibody at 1:50 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) SUR1 Antibody (D) Composite.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Gene Details
− No NCBI Gene data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_037171.2 |
|---|
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
ICC
Immunocytochemistry
SUR1 Antibody (orb67497)
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