SUR1 Antibody

• Catalog Number: orb67497
• Category: Antibodies
• Description: Mouse Anti-Rat SUR1 Monoclonal IgG1
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: N289/16 (Formerly sold as S289-16)
• Tested applications: ICC, IF, IHC, WB
• Reactivity: Hamster, Human, Mouse, Rat
• Isotype: IgG1
• Immunogen: Fusion protein amino acids 1548-1582 (cytoplasmic C-terminus) of rat SUR1
• Concentration: 1 mg/ml
• Dilution range: WB (1:1000), IHC-P (1:1000), ICC/IF (1:100)
• Conjugation: Unconjugated
• MW: 160kDa
• Target: SUR1
• Entrez: 25559
• UniProt ID: Q09429
• NCBI: NP_037171.2
• Storage: -20°C
• Buffer/Preservatives: PBS pH 7.4, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated
• Alternative names: AM60008PU-N Antibody, ABC36 Antibody, Abcc8 Antibo
• Note: For research use only
• Application notes: 1 µg/ml of SMC-409 was sufficient for detection of SUR1 in 20 µg of mouse brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
• Expiration Date: 12 months from date of receipt.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SUR1 Monoclonal Antibody, Clone N289/16. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-SUR1 Monoclonal Antibody at 1:50 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) SUR1 Antibody (D) Composite.