SUR1/ABCC8 Antibody

SKU: orb19968

Description

Goat polyclonal antibody to ABCC8

Research Area

Cancer, Epigenetics, Neuroscience

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:8000, WB: 0.5-1.5 μg/ml
• Reactivity: Human
• Predicted Reactivity: Canine, Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx 170kDa band observed in Human Brain (Cerebellum) lysates (calculated MW of 177kDa according to NP_000343.2). Recommended concentration: 0.5-1.5 μg/ml. An additional band of unknown identity was also consistently observed at 23kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: SUR1 / ABCC8
• Protein Sequence: EFDKPEKLLSRKD
• Molecular Weight: 177
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti ABCC8 antibody, anti SUR1 antibody, anti ATP-binding cassette, sub-family C (CFTR/MRP), member 8 antibody, anti ABC36 antibody, anti HHF1 antibody, anti HI antibody, anti HRINS antibody, anti MRP8 antibody, anti PHHI antibody, anti SUR antibody, anti TNDM2 antibody, anti ATP-binding cassette, sub-family C, member 8 antibody, anti sulfonylurea receptor (hyperinsulinemia) antibody

orb19968 (0.5ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

SUR1 / ABCC8

NCBI Gene ID

6833