SPTA1 Antibody

• Catalog Number: orb1260963
• Category: Antibodies
• Description: SPTA1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 940-1160 of human SPTA1 (NP_003117.2).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IF: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 300kDa
• Target: SPTA1
• UniProt ID: P02549
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: EL2, HPP, HS3, SPH3, SPTA, spectrin alpha chain, e
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IF: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using SPTA1 antibody (orb1260963) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Immunofluorescence analysis of rat bone marrow cells using SPTA1 antibody (orb1260963) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of mouse lung cells using SPTA1 antibody (orb1260963) at dilution of 1:100. Blue: DAPI for nuclear staining.