Spectrin alpha 1 Antibody / SPTA1

SKU: orb606619

Description

Spectrin is an actin crosslinking and molecular scaffold protein that links the plasma membrane to the actin cytoskeleton, and functions in the determination of cell shape, arrangement of transmembrane proteins, and organization of organelles. Spectrins function as membrane organizers and stabilizers, composed of non-homologous and chains, which aggregate side-to-side in an anti-parallel fashion to form dimers, tetramers and higher polymers. Spectrin Ia and spectrin Ib are present in erythrocytes, whereas spectrin IIa (also designated fodrin alpha) and spectrin IIb (also designated fodrin beta) are present in other somatic cells. The spectrin tetramers in erythrocytes act as barriers to lateral diffusion, but spectrin dimers seem to lack this function.

Research Area

Musculoskeletal & Connective Tissue Research

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 2-4ug/ml (order BSA/azide-free format),Western blot: 0.5-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Reactivity: Human
• Application Notes:
  Optimal dilution of the Spectrin alpha 1 antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2b, kappa
• Clone No.: SPTA1/1832
• Immunogen: A portion of amino acids 356-475 from the human protein was used as the immunogen for the Spectrin alpha 1 antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

UniProt

P02549