SPTA1 Antibody / Spectrin alpha 1

• Catalog Number: orb534493
• Category: Antibodies
• Description: Spectrin is an actin crosslinking and molecular scaffold protein that links the plasma membrane to the actin cytoskeleton, and functions in the determination of cell shape, arrangement of transmembrane proteins, and organization of organelles. Spectrins function as membrane organizers and stabilizers, composed of non-homologous and chains, which aggregate side-to-side in an anti-parallel fashion to form dimers, tetramers and higher polymers. Spectrin Ia and spectrin Ib are present in erythrocytes, whereas spectrin IIa (also designated fodrin alpha) and spectrin IIb (also designated fodrin beta) are present in other somatic cells. The spectrin tetramers in erythrocytes act as barriers to lateral diffusion, but spectrin dimers seem to lack this function.
• Species/Host: Mouse
• Clonality: Recombinant
• Clone Number: rSPTA1/1832
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: A portion of amino acids 356-475 from the human protein was used as the immunogen for the recombinant Spectrin alpha 1 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT (1)
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This recombinant Spectrin alpha 1 antibody is available for research use only.
• UniProt ID: P02549
• Storage: Store the recombinant Spectrin alpha 1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the recombinant Spectrin alpha 1 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human placenta with Spectrin alpha 1 antibody (clone rSPTA1/1832). Required HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

IHC staining of FFPE human placenta with Spectrin alpha 1 antibody (clone rSPTA1/1832). Required HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

Western blot testing of human Hela and K562 cell lysate with recombinant Spectrin alpha 1 antibody (clone rSPTA1/1832). Expected molecular weight: 260-280 kDa.

SDS-PAGE analysis of purified, BSA-free Spectrin alpha 1 antibody (clone rSPTA1/1832) as confirmation of integrity and purity.