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Catalog Number | orb639557 |
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Category | Antibodies |
Description | Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency. May function as a switch in neuronal development. Downstream SRRT target that mediates the promotion of neural stem cell self-renewal (By similarity). Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation. [UniProt] |
Species/Host | Rabbit |
Clonality | Recombinant |
Clone Number | SBX2-1R |
Tested applications | IHC-P, WB |
Reactivity | Human |
Isotype | Rabbit IgG, kappa |
Immunogen | Amino acids 176-305 were used as the immunogen for the recombinant SOX2 antibody. |
Antibody Type | Primary Antibody |
Dilution range | ELISA (order BSA-free format for coating),Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml |
Purity | Protein A affinity chromatography |
Conjugation | Unconjugated |
Formula | 0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide |
Hazard Information | This recombinant SOX2 antibody is available for research use only. |
UniProt ID | P48431 |
Storage | Store the recombinant SOX2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide). |
Buffer/Preservatives | 0.2 mg/ml with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide |
Note | For research use only |
Application notes | Optimal dilution of the antibody should be determined by the researcher. |
Expiration Date | 12 months from date of receipt. |
IHC staining of FFPE human cervical carcinoma with recombinant SOX2 antibody (clone SBX2-1R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
Western blot testing of NCCIT lysate with recombinant SOX2 antibody (clone SBX2-1R). Predicted molecular weight ~34 kDa.
IHC, WB | |
Bovine, Canine, Equine, Goat, Porcine, Rabbit, Rat, Sheep, Zebrafish | |
Frog, Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |