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Anti-SOX2 Antibody

Catalog Number: orb382052

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb382052
CategoryAntibodies
DescriptionRabbit polyclonal antibody to SOX2.
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, IH, WB
ReactivityHuman, Mouse, Rat
ImmunogenRecombinant full length protein of human SOX2
Antibody TypePrimary Antibody
Dilution rangeWB: 1:500-2000, IHC-P: 1:50-200, IF/ICC: 1:50-200
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ConjugationUnconjugated
TargetSOX2
Entrez20674, 6657
UniProt IDP48432, P48431
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative namesTranscription factor SOX-2
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NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-SOX2 Antibody

Western blot analysis of SOX2 expression in PC3 (A) whole cell lysates. (Predicted band size: 34 kD; Observed band size: 35 kD)

Anti-SOX2 Antibody

Immunohistochemical analysis of SOX2 staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-SOX2 Antibody

Immunofluorescent analysis of SOX2 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

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    IHC-P,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    100 μg
  • Anti-SOX2 Antibody [orb1473888]

    FC,  IF,  IH,  WB

    Human

    Mouse

    Monoclonal

    Unconjugated

    200 μl, 50 μl, 100 μl
  • SOX2 Antibody [orb242049]

    ELISA,  IF,  IHC,  WB

    Human, Mouse

    Rabbit

    Polyclonal

    Unconjugated

    50 μg, 100 μg
  • Sox2 Antibody [orb653349]

    FC,  ICC,  WB

    Mouse

    Mouse

    Monoclonal

    Unconjugated

    100 μl
  • Anti-SOX2 Antibody [orb411836]

    IF,  IH,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    100 μl, 200 μl, 50 μl