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Anti-SOX2 Antibody

Catalog Number: orb1473888

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb1473888
CategoryAntibodies
DescriptionMouse monoclonal antibody to SOX2
Species/HostMouse
ClonalityMonoclonal
Tested applicationsFC, IF, IH, WB
ReactivityHuman
ImmunogenRecombinant fusion protein of human SOX2. The exact sequence is proprietary.
Antibody TypePrimary Antibody
Dilution rangeWB (1/500 - 1/1000), IH (1/50 - 1/200), IF/IC (1/10 - 1/50), FC (1/10 - 1/50)
Form/AppearanceMouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ConjugationUnconjugated
TargetSOX2
Entrez6657
UniProt IDP48431
SourceMouse
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesMouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative namesTranscription factor SOX-2
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NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-SOX2 Antibody

Western blot analysis of SOX2 expression in 293 (A), transiently transfected with the SOX2 (B) whole cell lysates. (Predicted band size: 34 kD; Observed band size: 34 kD)

Anti-SOX2 Antibody

Immunohistochemical analysis of SOX2 staining in human lung carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-SOX2 Antibody

Immunofluorescent analysis of SOX2 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF555 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Anti-SOX2 Antibody

Flow cytometric analysis of NCIH460 cells using Anti-SOX2 Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Mouse IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.

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    IHC-P,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    100 μg
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    ELISA,  IF,  IHC,  WB

    Human, Mouse

    Rabbit

    Polyclonal

    Unconjugated

    50 μg, 100 μg
  • Anti-SOX2 Antibody [orb382052]

    IF,  IH,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    200 μl, 100 μl, 50 μl
  • Sox2 Antibody [orb653349]

    FC,  ICC,  WB

    Mouse

    Mouse

    Monoclonal

    Unconjugated

    100 μl
  • Anti-SOX2 Antibody [orb411836]

    IF,  IH,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    100 μl, 200 μl, 50 μl