SOX2 Antibody

• Catalog Number: orb1939275
• Category: Antibodies
• Description: Purified Mouse Monoclonal Antibody (Mab)
• Target: SOX2 recombinant protein is used to produce this monoclonal antibody.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG1
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• UniProt ID: P48431
• MW: 34310 Da
• Tested applications: FC, IF, IHC-P, WB
• Dilution range: IF: 1:100, IF: 1:10~50, WB: 1:200~2000, WB: 1:200~2000, WB: 1:1000, WB: 1:4000, IHC-P: 1:50~100
• Clone Number: 57CT23.3.4
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Transcription factor SOX-2, SOX2
• Note: For research use only
• NCBI: NP_003097.1

Fluorescent confocal image of SY5Y cells stained with SOX2 Antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with SOX2 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h).

Fluorescent image of A549 cell stained with SOX2 Antibody. A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SOX2 primary antibody (1:25, 1 h at 37°C. For secondary antibody, Alexa Fluor 488 conjugated donkey anti-mouse antibody (green) was used (1:400, 50 min at 37°C.Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C.SOX2 immunoreactivity is localized to Nucleus significantly.

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with SOX2 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot analysis of lysate from NCCIT cell line, using SOX2 Antibody. diluted at 1:1000. A goat anti-mouse IgG H&L (HRP) at 1:3000 dilution was used as the secondary Antibody. Lysate at 20 μg.

Western blot analysis of lysate from SOX2 protein, using SOX2 Antibody. diluted at 1:4000. A goat anti-mouse IgG H&L (HRP) at 1:3000 dilution was used as the secondary Antibody. Lysate at 20 μg.

Western blot analysis of SOX2 (arrow) using mouse monoclonal SOX2 Antibody. 293 cell lysates (2 μg/lane) either nontransfected (Lane 1) or transiently transfected with the SOX2 gene (Lane 2)

Western blot analysis of SOX2 Antibody by SOX2 recombinant protein. SOX2 (arrow) was detected using the purified Mab.