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SOX2 Rabbit Polyclonal Antibody

Catalog Number: orb500791

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb500791
CategoryAntibodies
DescriptionSOX2 Rabbit Polyclonal Antibody
TargetSOX2
ClonalityPolyclonal
Species/HostRabbit
IsotypeIgG
ConjugationUnconjugated
ReactivityMouse, Rat
Predicted ReactivityBovine, Canine, Equine, Gallus, Human, Sheep
Form/AppearanceLiquid
Concentration1mg/ml
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
ImmunogenKLH conjugated synthetic peptide derived from human SOX2 (227-317/317aa)
UniProt IDP48431
MW34 kDa
Tested applicationsIF, IHC-Fr, IHC-P
Dilution rangeIHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Antibody TypePrimary Antibody
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namestranscriptional factor SOX2; ANOP3; cb236; delta E
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NoteFor research use only
Expiration Date12 months from date of receipt.
SOX2 Rabbit Polyclonal Antibody

Blank control: Hela. Primary Antibody (green line): Rabbit Anti-SOX2 antibody (orb500791), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647, dilution: 1 µg/Test. Protocol The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

SOX2 Rabbit Polyclonal Antibody

Blank control: U251. Primary Antibody (green line): Rabbit Anti-SOX2 antibody (orb500791), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

SOX2 Rabbit Polyclonal Antibody

Cell: NCCIT, Concentration: 1:100, Host/Isotype: Rabbit/IgG, Flow cytometric analysis of Rabbit IgG isotype control (orb500791) on NCCIT (green) compared with control in the absence of primary antibody (blue) followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG (H+L) secondary antibody.

SOX2 Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Mouse embryo), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (SOX2) Polyclonal Antibody, Unconjugated (orb500791) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

SOX2 Rabbit Polyclonal Antibody

Sample: Cerebrum (Mouse) Lysate at 40 ug, Primary: Anti-SOX2 (orb500791) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 34 kD, Observed band size: 34 kD.

SOX2 Rabbit Polyclonal Antibody

Sample: Eye (Mouse) Lysate at 40 ug, Primary: Anti-SOX2 (orb500791) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 34 kD, Observed band size: 34 kD.

SOX2 Rabbit Polyclonal Antibody

Sample: Eye (Rat) Lysate at 40 ug, Primary: Anti-SOX2 (orb500791) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 34 kD, Observed band size: 34 kD.

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