SOX2 Antibody

• Catalog Number: orb1240008
• Category: Antibodies
• Description: SOX2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, IHC-P, WB
• Predicted Reactivity: Equine, Gallus, Porcine, Sheep
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: SOX2 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human SOX.The immunogen is located within the first 50 amino acids of SOX2.
• Concentration: 1 mg/mL
• Dilution range: SOX2 antibody can be used for detection of SOX2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL and immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: SOX2
• UniProt ID: P48431
• NCBI: NP_003097
• Storage: SOX2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: SOX2 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: SOX2 Antibody: ANOP3, MCOPS3, Transcription factor
• Note: For research use only
• Application notes: SOX2 antibody can be used for detection of SOX2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL and immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of SOX2 in 3T3 cell lysate with SOX2 antibody at (A) 1 and (B) 2 µg/mL.

Immunocytochemistry of SOX2 in 3T3 cells with SOX2 antibody at 5 µg/mL.

Immunofluorescence of SOX2 in 3T3 cells with SOX2 antibody at 20 µg/mL.

Immunohistochemistry of SOX2 in mouse brain tissue with SOX2 Antibody at 5 µg/mL.

Immunofluorescence of SOX2 in mouse brain tissue with SOX2 Antibody at 20 µg/mL. Green: SOX2 antibody (orb1240008) Red: Phylloidin staining Blue: DAPI staining.