SOX10 Antibody

SKU: orb2637366

Description

SOX-10 is a sensitive marker of melanoma, including conventional, spindled, and desmoplastic subtypes. It is expressed by metastatic melanomas and nodal capsular nevus in sentinel lymph nodes, but not by other lymph node components such as dendritic cells, which usually express S100 protein. Commonly used melanoma markers, such as anti-HMB-45 and anti-Melan-A, are poorly expressed in desmoplastic melanomas while SOX-10 is moderately to strongly expressed in desmoplastic melanomas. SOX-10 is considered as a very reliable marker for recognizing residual desmoplastic melanomas. In normal tissues, it is expressed in Schwann cells, melanocytes, and myoepithelial cells of salivary, bronchial and mammary glands. SOX-10 expression is also observed in mast cells.

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA (For coating purchase antibody without BSA),Western blot: 1-2ug/ml
• Reactivity: Human
• Application Notes:
  Optimal dilution of the SOX10 antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: SOX10/992
• Immunogen: A portion of amino acids 115-269 was used as the immunogen for the SOX10 antibody.
• Purification: Protein A/G affinity
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Western blot testing of human COLO-38 cell lysate using SOX10 antibody (clone SOX10/992). Expected molecular weight: 50-58 kDa.

Western blot analysis of SOX10 (A) recombinant protein and (B) human A375 cell lysate using SOX10 antibody (clone SOX10/992). Expected molecular weight: 50-58 kDa.

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using SOX10 antibody (clone SOX10/992). These results demonstrate the foremost specificity of the SOX10/992 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free SOX10 antibody (SOX10/992) as confirmation of integrity and purity.

Quick Database Links

UniProt

P56693