SOX10 Antibody (Center)

• Catalog Number: orb1930327
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Target: This SOX10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 231-259 amino acids of human SOX10.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Porcine
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: P56693
• MW: 49911 Da
• Tested applications: IHC-P, WB
• Dilution range: WB: 1:2000, WB: 1:1000, IHC-P: 1:25, IHC-P: 1:25, IHC-P: 1:10~50
• Antibody Type: Primary Antibody
• Clone Number: RB21835
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Transcription factor SOX-10, SOX10
• Note: For research use only
• NCBI: NP_008872.1

Staining SOX10 in human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Staining SOX10 in human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

SOX10 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SOX10 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

SOX10 Antibody (Center) western blot analysis in mouse heart tissue lysates (15 ug/lane). This demonstrates the SOX10 antibody detected SOX10 protein (arrow).

All lanes: Anti-SOX10 Antibody (Center) at 1:2000 dilution. Lane 1: A375 whole cell lysates. Lane 2: C6 whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 50 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.