SOX10 Antibody (Center)

SKU: orb1930327

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Cancer Biology

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB - 1:2000, IHC-P - 1:25
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Porcine

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB21835
• Immunogen: This SOX10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 231-259 amino acids of human SOX10. Antigen Region: 231-259 aa.
• Target: SOX10
• Molecular Weight: 49911 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Transcription factor SOX-10, SOX10

Staining SOX10 in human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Staining SOX10 in human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

SOX10 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SOX10 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

SOX10 Antibody (Center) western blot analysis in mouse heart tissue lysates (15 ug/lane). This demonstrates the SOX10 antibody detected SOX10 protein (arrow).

All lanes: Anti-SOX10 Antibody (Center) at 1:2000 dilution. Lane 1: A375 whole cell lysates. Lane 2: C6 whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 50 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

SOX10

UniProt

P56693

RefSeq (Protein):NP_008872.1