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Description
Research Area
Stem Cell & Developmental Biology
Images & Validation
−| Tested Applications | ELISA, IHC, IHC-P, WB |
|---|---|
| Dilution Range | IHC, IHC-P (5 - 10 µg/ml), Peptide-ELISA (1:10000), WB (1:500 - 1:1000) |
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Synthetic peptide from human SMO. (AA Range: 68-117) |
| Target | SMO / Smoothened |
| Purification | Immunoaffinity purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS (without Mg2+, Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide, 50% Glycerol |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−SMO, Frizzled family member 11, FZD11, Gx, Protein Gx, SMOH, Smoothened, Smoothened homolog
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Quick Database Links
Gene Symbol
SMO / Smoothened
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IHC-P
Immunohistochemistry Paraffin
ELISA
Enzyme-linked Immunosorbent Assay (EIA)









