SMO/Smoothened Antibody

SKU: orb1533807

Description

SMO/Smoothened Antibody

Research Area

Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: ICC, IF, IHC, IHC-P
• Dilution Range: ICC, IF, IHC, IHC-P (10 µg/ml)
• Reactivity: Bovine, Canine, Equine, Hamster, Human, Mammal, Monkey, Mouse, Porcine, Rabbit, Rat
• Application Notes:
  Further information: Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 10 ug/ml.

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Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic 15 amino acid peptide from N-Terminus extracellular domain of human SMO. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Bovine, Dog, Bat, Hamster, Elephant, Panda, Horse, Rabbit, Pig, Platypus (100%); Opossum (93%); Turkey, Chicken, Xenopus (87%); Zebrafish, Seq squirt (80%).
• Target: SMO / Smoothened
• Purification: Immunoaffinity purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS, 0.1% Sodium Azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

SMO, Frizzled family member 11, FZD11, Gx, Protein Gx, SMOH, Smoothened, Smoothened homolog

Quick Database Links

Gene Symbol

SMO / Smoothened