SMO/Smoothened Antibody

• Catalog Number: orb1533807
• Category: Antibodies
• Description: SMO/Smoothened Antibody
• Target: SMO / Smoothened
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Bovine, Canine, Equine, Hamster, Human, Mammal, Monkey, Mouse, Porcine, Rabbit, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS, 0.1% Sodium Azide. PBS, 0.1% Sodium Azide
• Immunogen: Synthetic 15 amino acid peptide from N-Terminus extracellular domain of human SMO. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Bovine, Dog, Bat, Hamster, Elephant, Panda, Horse, Rabbit, Pig, Platypus (100%); Opossum (93%); Turkey, Chicken, Xenopus (87%); Zebrafish, Seq squirt (80%).
• Tested applications: ICC, IF, IHC, IHC-P
• Dilution range: ICC, IF, IHC, IHC-P (10 µg/ml)
• Application notes: Further information: Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 10 ug/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: SMO, Frizzled family member 11, FZD11, Gx, Protein
• Note: For research use only

Human Kidney: Formalin-Fixed Paraffin-Embedded (FFPE)