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SMO Antibody

Catalog Number: orb1257903

DispatchUsually dispatched within 5-10 working days
$ 800.00
Catalog Numberorb1257903
CategoryAntibodies
DescriptionSMO Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, IHC, WB
ReactivityHuman, Mouse, Rat
IsotypeIgG
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 554-787 of human SMO (NP_005622.1).
Antibody TypePrimary Antibody
Concentrationbatch dependent
Form/AppearanceLiquid
ConjugationUnconjugated
MWObserved: 86kDa
TargetSMO
UniProt IDQ99835
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid
Alternative namesSMO, smoothened homolog (Drosophila), HGNC:11119,
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NoteFor research use only
Application notesWB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100
Expiration Date12 months from date of receipt.
SMO Antibody

Western blot analysis of extracts of various cell lines, using SMO antibody (orb1257903) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

SMO Antibody

Immunohistochemistry of paraffin-embedded rat brain using SMO antibody (orb1257903) (40x lens).

SMO Antibody

Immunofluorescence analysis of C6 cells using SMO Polyclonal Antibody (orb1257903) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

SMO Antibody

Immunofluorescence analysis of HeLa cells using SMO Polyclonal Antibody (orb1257903) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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