SMO Rabbit Polyclonal Antibody

SKU: orb5757

Description

SMO Rabbit Polyclonal Antibody

Research Area

Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: WB
• Dilution Range: WB=1:500-2000
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine, Equine, Rabbit, Rat, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human SMO (551-650/787aa)
• Target: SMO
• Molecular Weight: 84 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CRJS; FZD11; Gx; PHLS; SMOH; E130215L21Rik; bnb; smoothened; SMO_HUMAN; SMO; Protein Gx; SMO_MOUSE; SMO_RAT;

Paraformaldehyde-fixed, paraffin embedded (human pancreatic cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Smoothened) Polyclonal Antibody, Unconjugated (orb5757) at 1:2000 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Quick Database Links

Gene Symbol

SMO

UniProt

Q99835