SHH Antibody

• Catalog Number: orb97480
• Category: Antibodies
• Description: Mouse monoclonal antibody to SHH
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 5H4A9
• Tested applications: FC, IHC, WB
• Reactivity: Human, Monkey, Mouse
• Isotype: Mouse IgG1
• Immunogen: Purified recombinant fragment of human SHH (AA: 26-161) expressed in E. Coli.
• Antibody Type: Primary Antibody
• Concentration: Approximately 1mg/ml (varies from batch to batch). Please inquire for precise concentration.
• Dilution range: WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000; FCM: 1/200 - 1/400; ELISA: 1/10000
• Conjugation: Unconjugated
• MW: 49.6kDa
• Target: SHH
• Entrez: 6469
• UniProt ID: Q15465
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Purified antibody in PBS with 0.05% sodium azide
• Alternative names: TPT; HHG1; HLP3; HPE3; SMMCI; TPTPS; MCOPCB5
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometric analysis of HeLa cells using SHH antibody

Western blot analysis of LNCaP (Lane1), HepG2 (Lane2), PANC-1 (Lane3)

HeLa (Lane4), SK-N-SH (Lane5), F9 (Lane6), NIH3T3 (Lane7), and COS7 (Lane8) cell lysate using SHH antibody

Western blot analysis of human recombinant protein using SHH antibody

Line graph illustrates about the Ag-Ab reactions using different concentrations of antigen and serial dilutions of SHH antibody

Western blot analysis of HEK293 (Lane1) and SHH (AA: 26-161)-hIgGFc transfected HEK293 (Lane2) cell lysate using SHH antibody