SHH Antibody

SKU: orb97480

Description

Mouse monoclonal antibody to SHH

Images & Validation

• Tested Applications: FC, IHC, WB
• Dilution range: WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000; FCM: 1/200 - 1/400; ELISA: 1/10000
• Reactivity: Human, Monkey, Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone No.: 5H4A9
• Immunogen: Purified recombinant fragment of human SHH (AA: 26-161) expressed in E. Coli.
• Target: SHH
• Molecular Weight: 49.6kDa

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Purified antibody in PBS with 0.05% sodium azide
• Concentration: Approximately 1mg/ml (varies from batch to batch). Please inquire for precise concentration.
• Disclaimer: For research use only

Alternative Names

TPT; HHG1; HLP3; HPE3; SMMCI; TPTPS; MCOPCB5

Flow cytometric analysis of HeLa cells using SHH antibody

Western blot analysis of LNCaP (Lane1), HepG2 (Lane2), PANC-1 (Lane3),HeLa (Lane4), SK-N-SH (Lane5), F9 (Lane6), NIH3T3 (Lane7), and COS7 (Lane8) cell lysate using SHH antibody

Western blot analysis of human recombinant protein using SHH antibody

Line graph illustrates about the Ag-Ab reactions using different concentrations of antigen and serial dilutions of SHH antibody

Western blot analysis of HEK293 (Lane1) and SHH (AA: 26-161)-hIgGFc transfected HEK293 (Lane2) cell lysate using SHH antibody

Immunohistochemical analysis of formalin fixed and paraffin-embedded liver cancer tissues using SHH antibody

Quick Database Links

Gene Symbol

SHH

UniProt

Q15465

NCBI Gene ID

6469