Sheep anti Mouse IgG2a (1b allotype specific)

• Catalog Number: orb22109
• Category: Antibodies
• Description: Purified IgG fraction of polyclonal sheep antiserum to Mouse IgG2a(1b), allotype specific. As unlabelled primary or secondary reagent for indirect detection of IgG2a(1b) at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to prepare conjugates of the user's own choice; to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established by titration before being used. Typical working dilutions in histochemistry are usually between 1:100 and 1:250; in ELISA and comparable non-precipitating antibody-binding assays between 1:500 and 1:4.000. These data should be interpreted as general recommendations only.
• Species/Host: Sheep
• Clonality: Polyclonal
• Antibody Type: Secondary Antibody
• Form/Appearance: Purified hyperimmune IgG fraction, lyophilized from a solution in phosphate buffered saline (pH7.2). No preservative added, as it may interfere with the antibody activity.
• Conjugation: Unconjugated
• Hazard Information: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Biorbyt accepts no liability for any inaccuracies or omissions in this information.
• Source: Highly purified normal homogenous IgG2a(1b) isolated from mouse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
• RRID: AB_10751594
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Purified hyperimmune IgG fraction, lyophilized from a solution in phosphate buffered saline (pH7.2). No preservative added, as it may interfere with the antibody activity.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.