Sheep Mouse IgG2a (1b allotype specific), conjugated with Biotin Antibody

SKU: orb22107

Description

Biotin-conjugated IgG fraction of polyclonal sheep antiserum to Mouse IgG2a(1b), allotype specific. In immunocytochemical and immunohistochemical staining for the detection of IgG2a(1b) at the cellular and subcellular level. In non-isotopic assay methodology (e.g. ELISA) to identify and measure IgG2a(1b) in mouse serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user's choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:50 and 1:200; in ELISA and comparable non-precipitating antibody-binding assays between 1:500 and 1:2,000. These data should be interpreted as general recommendations only.

Images & Validation

• Reactivity: Mouse

Key Properties

• Antibody Type: Secondary Antibody
• Host: Sheep
• Clonality: Polyclonal
• Conjugation: Biotin

Storage & Handling

• Storage: The lyophilized immunoconjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
• Form/Appearance: Biotin-coupled purified hyperimmune IgG fraction, lyophilized from a solution in phosphate buffered saline (pH7.2). No preservative added, as it may interfere with the antibody activity.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only