Sheep Mouse IgG2a (1b allotype specific), conjugated with Horseradish peroxidase Antibody

SKU: orb22104

Description

Horseradish peroxidase-conjugated IgG fraction of polyclonal sheep antiserum to Mouse IgG2a(1b), allotype specific. In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgG2a(1b) at the cellular an subcellular level. In non-isotopic assay methodology (e.g. ELISA and Western blotting) to identify and measure IgG2a(1b) in mouse serum or other body fluids. This immunoconjugate is not pre-diluted. Its optimum working dilution should be established by titration. Excess labelled antibody may by itself cause high unspecific background staining or interfere with the specific signal. Working dilutions fro histochemical and cytochemical use are usually between 1:50 and 1:200; in ELISA and comparable non-precipitating antibody-binding assays between 1:500 and 1:2,000. These data should be interpreted as general recommendations only.

Images & Validation

• Reactivity: Mouse

Key Properties

• Antibody Type: Secondary Antibody
• Host: Sheep
• Clonality: Polyclonal
• Conjugation: HRP

Storage & Handling

• Storage: The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
• Form/Appearance: Horseradish peroxidase-coupled hyperimmune IgG fraction, lyophilized from a solution in phosphate buffered saline (pH7.2). No preservative added, as it may interfere with the antibody activity.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only