HTR3A Antibody

SKU: orb1246966

Description

HTR3A Antibody

Research Area

Neuroscience

Images & Validation

• Tested Applications: ELISA, ICC, IF, IHC, WB
• Reactivity: Human, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 55kDa band observed in Human Colon lysates (calculated MW of 55.3kDa according to NP_000860.2. Recommended concentration: 0.3-1µg/mL. Primary incubation was 1 hour. An additional band of unknown identity was also consistently observed at 200kDa. This band was successfully blocked by incubation with the immunising peptide.Immunohistochemistry:Paraffin embedded Human Small Intestine. Recommended concentration: 3.75µg/mL. ICC/ IF:This product has been successfully used in IF/ ICC on Rat: Lu CL et al, Gastroenterology. 2009 Sep;137(3):1040-50. PMID: 19344720.

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Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-GPQDFEKSPRDR
• Target: HTR3A
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

HTR3A, serotonin receptor, 5-hydroxytryptamine (serotonin) receptor 3A, 5-HT-3, 5-HT3A, 5-HT3R, 5HT3R, HTR3, 5-hydroxytryptamine (serotonin) receptor-3, 5HT3 serotonin receptor, Serotonin-gated ion channel receptor, truncated receptor, containing only 3 transmembrane domains

Quick Database Links

Gene Symbol

HTR3A

UniProt

P46098

RefSeq (Protein):NP_001155244.1, NP_000860.2, NP_998786.2