Serotonin receptor 3A/HTR3A Antibody

SKU: orb19303

Description

Goat polyclonal antibody to HTR3A

Images & Validation

• Tested Applications: ELISA, FC, ICC, IHC
• Dilution range: ELISA: 1:128000, IHC-P: 3.75 μg/ml
• Reactivity: Human, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 55kDa band observed in Human Colon lysates (calculated MW of 55.3kDa according to NP_000860.1. Recommended concentration: 0.3-1 μg/ml. An additional band of unknown identity was also consistently observed at 200kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: Serotonin receptor 3A / HTR3A
• Molecular Weight: 58.4 55.3 53.8
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti HTR3A antibody, anti serotonin receptor antibody, anti 5-hydroxytryptamine (serotonin) receptor 3A antibody, anti 5-HT-3 antibody, anti 5-HT3A antibody, anti 5-HT3R antibody, anti 5HT3R antibody, anti HTR3 antibody, anti 5-hydroxytryptamine (serotonin) receptor-3 antibody, anti 5HT3 serotonin receptor antibody, anti Serotonin-gated ion channel receptor antibody, anti truncated receptor, containing only 3 transmembrane domains antibody

orb19303 (0.3ug/ml) staining of Human Colon lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemical staining of Human Small Intestine using HTR3A antibody

Quick Database Links

Gene Symbol

Serotonin receptor 3A / HTR3A

NCBI Gene ID

3359