Goat anti-Serotonin receptor 3A / HTR3A Antibody

• Catalog Number: orb19303
• Category: Antibodies
• Description: Goat polyclonal antibody to HTR3A
• Target: Serotonin receptor 3A / HTR3A
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: GPQDFEKSPRDR
• RRID: AB_10747418
• MW: 58.4 55.3 53.8
• Tested applications: ELISA, FC, ICC, IHC
• Dilution range: ELISA: 1:128000, IHC-P: 3.75 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 55kDa band observed in Human Colon lysates (calculated MW of 55.3kDa according to NP_000860.1. Recommended concentration: 0.3-1 μg/ml. An additional band of unknown identity was also consistently observed at 200kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti HTR3A antibody, anti serotonin receptor antib
• Note: For research use only
• Entrez: 3359

orb19303 (0.3ug/ml) staining of Human Colon lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemical staining of Human Small Intestine using HTR3A antibody