SARS-CoV-2 (COVID-19) Spike S2 Antibody

• Catalog Number: orb1239031
• Category: Antibodies
• Description: SARS-CoV-2 (COVID-19) Spike S2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Virus
• Isotype: IgG
• Immunogen: Anti-SARS-CoV-2 (COVID-19) Spike antibody (orb1239031) was raised against a peptide corresponding to 16 amino acids near the center terminus of SARS-CoV-2 (COVID-19) Spike glycoprotein. The immunogen is located within 900-950 amino acids of SARS-CoV-2 (COVID-19) Spike protein.
• Concentration: 1 mg/mL
• Dilution range: WB: 1 μg/mL.Antibody validated: SARS-CoV-2 (COVID-19) Spike antibody can be used for the detection of SARS-CoV-2 (COVID-19) Spike protein in WB. It will detect 2 ng of free peptide at 1 μg/mL. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: S
• UniProt ID: P0DTC2
• NCBI: QHD43416
• Storage: SARS-CoV-2°COVID-19) Spike antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: SARS-CoV-2 (COVID-19) Spike Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: SARS-CoV-2 (COVID-19) Spike Antibody: Severe acute
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot Validation with SARS-CoV-2 (COVID-19) Spike S2 ECD Recombinant Protein. Loading: 30 ng per lane of SARS-CoV-2 (COVID-19) Spike S2 ECD recombinant protein, orb1231609. Antibodies: SARS-CoV-2 (COVID-19) Spike S2, orb1239031, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 1 µg/mL and Lane 2: 2 µg/mL.

ELISA Validation. Antibodies: SARS-CoV-2 (COVID-19) Spike S2 Antibody, orb1239079. A direct ELISA was performed using immunogen peptide (orb1231697) as coating antigen and the anti-SARS-CoV-2 (COVID-19) Spike S2 antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.3 ng/mL to 1000 ng/mL.